DIA Source

Free Vitamin D

Free 25OH Vitamin D Elisa (# KAPF1991)

KAPF1991The Free 25OH Vitamin D ELISA (# KAPF1991) is a competitive ELISA assay, based on patented monoclonal antibodies, that allows for the direct measurement of the free fraction of 25OH Vitamin D in serum. The assay is calibrated against a symmetric dialysis method. It shows excellent sensitivity, precision and performance characteristics, as performed according to the CLSI guidelines.

The assay is unique and exclusively available at DIAsource Immunoassays.


Download : IFU Free 25OH Vitamin D

Assay protocol :

  • Reagent preparation
  • 10 μL Reconstituted Calibrator, Control or sample
  • Incubate 90′ 37° shaking at 650 rpm
  • Wash 3 x 350 μL
  • 100 μL Working dilution Biot-VitD reagent
  • Incubate 30′ 37° shaking at 650 rpm
  • Wash 3 x 350 μL
  • 100 μL Streptavidin-HRP reagent
  • 90 μL Sample diluent
  • 100 μL Substrate reagent
  • 15′ Room temperature, stationary in dark
  • 100 μL Stop reagent
  • Read at 450 nm
  • Calculate results

Download : Assay Protocol – KAPF1991